Last Updated 15 July 2016
To establish acceptable methods of euthanasia for investigative personnel to perform. The current procedure is composed of two parts; a primary method followed by a secondary method to ensure death and/or prevent revival.
- Investigative Personnel
- Euthanasia: Term used to describe the ending the life of an individual animal in a way that minimizes or eliminates pain and distress.
- Methods of euthanasia must be in accordance with an IACUC approved animal use protocol.
- Methods described in this document are the most common forms of euthanasia. Contact Training Core for training on other euthanasia methods, ULAMfirstname.lastname@example.org.
Animal Consolidation Procedure for Inhalant Euthanasia
- Consolidate mice within the animal room.
- Maximum of 10 mice in a small mouse box.
- Maximum of 25 mice in a large mouse box.
- Provide ample space for animals to move and assume normal postures.
- Upon consolidation, animals are euthanized immediately (maximum of 10 minutes).
- Follow standard housing densities when consolidating other rodents (e.g. rats) for euthanasia.
Primary Euthanasia Method
- Animals must be observed and monitored at all times until death.
- It may take 5 minutes or more for animals to stop breathing during euthanasia.
- The most common method of adult and adolescent rodent (> 10 days old) euthanasia is exposure to carbon dioxide (CO2).
- Neonates (pinkies), without hair and <10 days old, are typically euthanized by decapitation. Inhalation anesthetics or CO2 exposure may or may not be used prior to decapitation.
- CO2 euthanasia is not as effective in neonates.
- If CO2 euthanasia is employed with neonates, a secondary method must be performed.
- Follow the Step by Step Euthanasia Procedures posted within the euthanasia room to euthanize animals.
Secondary Euthanasia Method
- Procedures must be employed to ensure death so that the animal cannot revive.
- The most commonly used method is bilateral pneumothorax.
- Using a scalpel blade, an incision is made in both sides of the rodent's chest cavity or through the diaphragm.
- UM Methods of Euthanasia by Species
- UCUCA Statement on Confirmation of Euthanasia via Rigor Mortis (No link to documents new location)
- UM Policy on Rodent Euthanasia via Carbon Dioxide Overdose
- Additional Information for Investigators and Staff Regarding Rodent Euthanasia via Carbon Dioxide Overdose